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ra1ar membranes  (Revvity)
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Revvity ra1ar membranes
Figure 1. (A) Functional activity of agonists 2 and 7, in stimulation of guanine nucleotide binding at the <t>rA1AR</t> (recombinant A1AR membrane preparations from CHO-K1 cells, PerkinElmer, compared to 2). (B) Effects of agonists 7 and 16 in inhibition of cAMP accumulation at hA3AR (in A3AR-expressing CHO cells, treated with 10 μM forskolin, compared to 16). 100% value is defined as effect of 1 μM 16. Also, functional assays at the hA1AR are shown for several derivatives (EC50 or IC50 in nM): stimulation of [35S]GTPγS binding (C, 9, 28.0 ± 9.0; 16, 0.12 ± 0.05; 40, 758 ± 175); inhibition of forskolin-stimulated cAMP production (D, 9, 0.14; 40, 87); β-arrestin2 recruitment (E, 9, 209 ± 90; 16, 5.03 ± 2.84; 40, 2460 ± 800).
Ra1ar Membranes, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ra1ar+membranes/10__1021_slash_acs__jmedchem__8b01662-483-14-16?v=Revvity
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ra1ar membranes - by Bioz Stars, 2026-07
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Figure 1. (A) Functional activity of agonists 2 and 7, in stimulation of guanine nucleotide binding at the rA1AR (recombinant A1AR membrane preparations from CHO-K1 cells, PerkinElmer, compared to 2). (B) Effects of agonists 7 and 16 in inhibition of cAMP accumulation at hA3AR (in A3AR-expressing CHO cells, treated with 10 μM forskolin, compared to 16). 100% value is defined as effect of 1 μM 16. Also, functional assays at the hA1AR are shown for several derivatives (EC50 or IC50 in nM): stimulation of [35S]GTPγS binding (C, 9, 28.0 ± 9.0; 16, 0.12 ± 0.05; 40, 758 ± 175); inhibition of forskolin-stimulated cAMP production (D, 9, 0.14; 40, 87); β-arrestin2 recruitment (E, 9, 209 ± 90; 16, 5.03 ± 2.84; 40, 2460 ± 800).

Journal: Journal of Medicinal Chemistry

Article Title: Design and in Vivo Characterization of A1 Adenosine Receptor Agonists in the Native Ribose and Conformationally Constrained (N)-Methanocarba Series

doi: 10.1021/acs.jmedchem.8b01662

Figure Lengend Snippet: Figure 1. (A) Functional activity of agonists 2 and 7, in stimulation of guanine nucleotide binding at the rA1AR (recombinant A1AR membrane preparations from CHO-K1 cells, PerkinElmer, compared to 2). (B) Effects of agonists 7 and 16 in inhibition of cAMP accumulation at hA3AR (in A3AR-expressing CHO cells, treated with 10 μM forskolin, compared to 16). 100% value is defined as effect of 1 μM 16. Also, functional assays at the hA1AR are shown for several derivatives (EC50 or IC50 in nM): stimulation of [35S]GTPγS binding (C, 9, 28.0 ± 9.0; 16, 0.12 ± 0.05; 40, 758 ± 175); inhibition of forskolin-stimulated cAMP production (D, 9, 0.14; 40, 87); β-arrestin2 recruitment (E, 9, 209 ± 90; 16, 5.03 ± 2.84; 40, 2460 ± 800).

Article Snippet: Functional assay at rA1AR was performed by GVK Biosciences, Hyderbad, India (Study No. 050-13-IVP). rA1AR membranes (PerkinElmer rat A1, 611051- 1400UA) were pretreated with adenosine deaminase at 1 U/mL.

Techniques: Functional Assay, Activity Assay, Binding Assay, Recombinant, Membrane, Inhibition, Expressing